Nipah virus (NiV) is a highly pathogenic zoonotic virus belonging to the Henipavirus genus of the Paramyxoviridae family. It was first identified during an outbreak in Malaysia in 1998–1999 and has since caused sporadic outbreaks in South and Southeast Asia. Nipah virus can spread from animals to humans, from humans to humans, and through contaminated food sources.
Because of its high mortality rate and lack of specific antiviral treatment, Nipah virus is classified as a priority pathogen by the World Health Organization (WHO).
How Nipah Virus Spreads
Nipah virus transmission occurs mainly through:
- Direct contact with infected bats (natural reservoir)
- Contact with infected pigs or other intermediate hosts
- Consumption of contaminated food (such as raw date palm sap)
- Human-to-human transmission via respiratory droplets or bodily fluids
Healthcare and laboratory workers are at increased risk without proper infection control and specimen handling procedures.
Common Symptoms of Nipah Virus Infection
Symptoms typically appear 4–14 days after exposure and may include:
- Fever and headache
- Muscle pain and vomiting
- Sore throat and cough
- Acute respiratory distress
- Encephalitis (inflammation of the brain)
- อาการชัก, coma, and potentially death
Early diagnosis is critical to patient management and outbreak control.
Laboratory Diagnosis Techniques for Nipah Virus
Accurate laboratory diagnosis of Nipah virus relies on well-controlled biosafety conditions and validated diagnostic methods.
RT-PCR Testing
ปฏิกิริยาลูกโซ่โพลีเมอเรสการถอดรหัสแบบย้อนกลับแบบเรียลไทม์ (อาร์ที-พีซีอาร์) is the most widely used method for detecting Nipah virus RNA during the acute phase of infection. It offers high sensitivity and specificity when performed on properly collected specimens.
ELISA and Serological Tests
Enzyme-linked immunosorbent assay (เอลิซา) is used to detect IgM and IgG antibodies against Nipah virus, particularly during later stages of infection or for surveillance purposes.
การแยกไวรัส
Virus isolation is conducted only in Biosafety Level 4 (BSL-4) laboratories due to the high risk associated with live Nipah virus handling. This method is mainly used for research and confirmation purposes.
Sample Collection and Handling for Nipah Virus Testing
Correct sample collection and handling are essential for reliable diagnostic results and biosafety.
Types of Clinical Specimens
Common specimens used for Nipah virus testing include:
- Oropharyngeal and nasopharyngeal swabs
- เลือด (serum or plasma)
- น้ำไขสันหลัง (ซีเอสเอฟ)
- Urine samples
แนวทางปฏิบัติที่ดีที่สุดสำหรับการรวบรวมตัวอย่าง
- Use sterile, individually packaged specimen collection swabs
- สวมใส่อุปกรณ์ป้องกันส่วนบุคคลที่เหมาะสม (อุปกรณ์ป้องกันส่วนบุคคล)
- Avoid contamination and ensure proper labeling
- Follow standard operating procedures (จิบ)
Sample Transport and Storage
- Place collected swabs immediately into viral transport medium (วี.ที.เอ็ม)
- Maintain cold-chain conditions (2–8°C) during transport
- Process samples as soon as possible or store at recommended temperatures
Recommended MEIDIKE GENE Specimen Collection Products
To ensure accurate Nipah virus testing and laboratory safety, MEIDIKE GENE offers a comprehensive range of high-quality specimen collection products suitable for viral diagnostics:
Flocked Oropharyngeal and Nasopharyngeal Swabs
MEIDIKE GENE sterile nylon flocked swabs provide excellent sample absorption and release, making them ideal for RT-PCR testing of Nipah virus and other respiratory pathogens.
สื่อการขนส่งไวรัส (วี.ที.เอ็ม) ชุดอุปกรณ์
MEIDIKE GENE VTM kits are designed to preserve viral RNA integrity during sample transport and storage, supporting reliable molecular testing results.
Complete Specimen Collection Kits
สำหรับห้องปฏิบัติการ, โรงพยาบาล, and public health agencies, MEIDIKE GENE also supplies customized specimen collection kits that include swabs, transport tubes, preservation solutions, and biohazard packaging.
Looking for reliable specimen collection solutions? please contact our sales team at info@medicoswab.com or visit our product page at https://www.medicoswab.com/products/
Prevention and Infection Control Measures
While no specific treatment exists for Nipah virus, prevention focuses on:
- Avoiding contact with infected animals
- ฝึกสุขอนามัยของมือที่ดี
- Using PPE in healthcare settings
- Implementing strict laboratory biosafety protocols
Early detection combined with proper specimen collection plays a key role in controlling outbreaks.
