Directrices para la recogida de muestras de viruela del mono

Se deben recolectar muestras de piel de las lesiones tan pronto como se sospeche de viruela simica.. La recolección adecuada de muestras es el paso más importante en el diagnóstico de laboratorio de enfermedades infecciosas.. Las muestras recolectadas incorrectamente pueden dar lugar a falsos negativos o resultados no concluyentes.. A continuación se presentan pautas para la recolección de muestras de viruela simica..

1. Remover 1 hisopo de recogida de muestras from its package. Do not touch the tip of the swab with your hands. Solo 1 swab per lesion is required for testing.
2. Unscrew the top of the collection tube. Hold the swab in 1 hand and the collection tube in the other.
3. Swab a pustule/lesion vigorously without unroofing or lancing the lesion.
4. Insert the swab into the viral transport medium without touching the outside of the collection tube, and break off the end of the swab, if required.
5. Screw the top of the collection tube back on tightly. Leaking specimens will be rejected.

Nota:

• Optimum personal protective equipment (EPP) for specimen collection is a gown,gloves, eye protection (e.g. goggles or a face shield that covers the front and sides of the face), and a NIOSH-approved particulate respirator equipped with N95 filters or higher.
• Specimens should be collected in a single-person room, with the door closed (if safe to do so). Special air handling is not required.
• Use a sterile nylon, poliéster, or Dacron swab with a plastic or thin aluminum shaft. Do not use other types of swabs. Do not use swabs contained within a media transport system (e.g. ESwab)
• Vigorously swab or brush lesion. If the skin atop the vesicle or pustule is intact, it may be necessary to gently lance and lift the lesion.
• Place each specimen in a separate sterile container