Saliva collection has become one of the most popular methods for obtaining DNA samples for genetic testing, molecular diagnostics, research studies, and biobanking. 採血と比べて, saliva sampling is non-invasive, 無痛, and suitable for self-collection.
Among the various saliva collection solutions available, split-design saliva collectors offer a convenient and reliable way to collect, preserve, and transport saliva specimens. By separating the collection tube and preservation solution before use, these devices help maintain reagent stability while ensuring easy activation during sample collection.
This guide explains how a split saliva collector works, how to collect a high-quality saliva sample, and how to store the specimen properly for optimal DNA preservation.
A split saliva collector is a saliva collection device designed with separate compartments for saliva collection and preservation solution storage.
通常, the device consists of:
Proper preparation is essential for obtaining a high-quality DNA sample.
To reduce contamination and improve sample quality:
These precautions help ensure that the collected saliva contains sufficient intact cells for DNA extraction.
Remove the saliva collector from its packaging.
使用する前, inspect the device carefully and ensure that:
Do not use the kit if any component appears damaged or contaminated.
Rinse your mouth thoroughly with clean water to remove food particles and debris.
すすぎ後, wait approximately 30 minutes before collecting the sample.
This waiting period helps prevent dilution or contamination of the specimen.
To improve DNA yield:
This process helps release exfoliated epithelial cells into the saliva, increasing the amount of DNA available for testing.
Spit saliva into the collection funnel slowly and naturally.
Continue until the liquid saliva level reaches the 2 mL fill line.
重要:
Only the liquid saliva should be measured. Bubbles and foam should not be counted as part of the required sample volume.
Hold the collector upright.
Carefully unscrew the cap of the preservation solution tube and slowly pour the solution into the collection funnel.
Keeping the collection tube upright, carefully remove the collection funnel by unscrewing it from the tube.
Avoid spilling any sample during this process.
Securely tighten the cap on the collection tube.
Gently invert the tube 5–6 times to ensure complete mixing of:
Once mixed, the sample is ready for storage and transportation.
After collection and mixing, proper storage is essential.
The stabilized saliva sample can be stored at room temperature for up to:
15 日々
This provides flexibility for sample transportation and laboratory submission.
For extended storage, keep samples refrigerated at:
2–8℃ (35.6–46.4°F)
Under these conditions, DNA stability can be maintained for up to:
3 月
最良の結果を得るために, samples should be shipped to the testing laboratory as soon as possible after collection.
To ensure accurate DNA testing results, avoid the following errors:
MEIDIKE GENE offers professionally designed split saliva collection devices for DNA preservation and molecular testing applications.
The split-design system helps ensure sample integrity while providing a simple and user-friendly collection experience.
