如何选择合适的病毒传输介质

选择合适的病毒传输介质(VTM) 对于保持病毒 RNA 的完整性和确保准确的测试结果至关重要. 常用的两种 VTM – 非灭活和灭活. 在本文中, 我们将讨论如何根据您的需要选择合适的病毒传输介质.

MEIDIKE GENE® VTM Kit

Inactivated Virus Transport Medium

It is mainly a virus lysis type preservation solution improved by nucleic acid extraction lysis solution. The high concentration of guanidine salt in the sample can effectively inactivate the virus, which can effectively prevent the operator from secondary infection, but it also contains Rnase inhibitor, which can protect the viral nucleic acid from being degraded, so that the subsequent detection by NT-PCR can be performed. . 而且, it can be stored at room temperature for a relatively long time, which saves the cost of virus sample storage and transportation.

Non-inactivated Virus Transport Medium

It is mainly a virus maintenance liquid type preservation solution improved on the basis of the transport medium. It can preserve the activity of the virus in vitro and the integrity of antigens and nucleic acids, protect the viral protein coat from being easily decomposed, and maintain the originality of the virus sample to a large extent. In addition to nucleic acid extraction and detection, this preservation solution can also be used for virus culture, isolation, and antigen detection, 等等。, but long-term storage after sampling requires strict low temperature.

Regardless of whether it is an inactivated type or a non-inactivated type, the virus sampling tube before sampling must be strictly inactivated and sterilized to ensure that there are no other microorganisms in the tube, which may cause the virus to decompose or other influences after sampling and cause false detection. After swab sampling, if a collection tool or preservation solution of poor quality is used, it will affect the subsequent test results and even cause misdiagnosis. 所以, you must choose a virus sampling tube produced by a professionally qualified manufacturer.