尼帕病毒: 原因, 症状, 诊断, 和样本采集指南

Nipah virus (NiV) is a highly pathogenic zoonotic virus belonging to the Henipavirus genus of the Paramyxoviridae family. It was first identified during an outbreak in Malaysia in 1998–1999 and has since caused sporadic outbreaks in South and Southeast Asia. Nipah virus can spread from animals to humans, from humans to humans, and through contaminated food sources.

Because of its high mortality rate and lack of specific antiviral treatment, Nipah virus is classified as a priority pathogen by the World Health Organization (WHO).

How Nipah Virus Spreads

Nipah virus transmission occurs mainly through:

• Direct contact with infected bats (natural reservoir)
• Contact with infected pigs or other intermediate hosts
• Consumption of contaminated food (such as raw date palm sap)
• Human-to-human transmission via respiratory droplets or bodily fluids

Healthcare and laboratory workers are at increased risk without proper infection control and specimen handling procedures.

Common Symptoms of Nipah Virus Infection

Symptoms typically appear 4–14 days after exposure and may include:

• Fever and headache
• Muscle pain and vomiting
• Sore throat and cough
• Acute respiratory distress
• Encephalitis (inflammation of the brain)
• 癫痫发作, coma, and potentially death

Early diagnosis is critical to patient management and outbreak control.

Laboratory Diagnosis Techniques for Nipah Virus

Accurate laboratory diagnosis of Nipah virus relies on well-controlled biosafety conditions and validated diagnostic methods.

RT-PCR Testing

实时逆转录聚合酶链式反应 (逆转录聚合酶链反应) is the most widely used method for detecting Nipah virus RNA during the acute phase of infection. It offers high sensitivity and specificity when performed on properly collected specimens.

ELISA and Serological Tests

Enzyme-linked immunosorbent assay (酶联免疫吸附试验) is used to detect IgM and IgG antibodies against Nipah virus, particularly during later stages of infection or for surveillance purposes.

病毒分离

Virus isolation is conducted only in Biosafety Level 4 (BSL-4) laboratories due to the high risk associated with live Nipah virus handling. This method is mainly used for research and confirmation purposes.

Sample Collection and Handling for Nipah Virus Testing

Correct sample collection and handling are essential for reliable diagnostic results and biosafety.

Types of Clinical Specimens

Common specimens used for Nipah virus testing include:

• Oropharyngeal and nasopharyngeal swabs
• 血 (serum or plasma)
• 脑脊液 (脑脊液)
• Urine samples

样本采集的最佳实践

• Use sterile, individually packaged specimen collection swabs
• 穿戴适当的个人防护装备 (个人防护装备)
• Avoid contamination and ensure proper labeling
• Follow standard operating procedures (SOP)

Sample Transport and Storage

• Place collected swabs immediately into viral transport medium (VTM)
• Maintain cold-chain conditions (2–8°C) during transport
• Process samples as soon as possible or store at recommended temperatures

Recommended MEIDIKE GENE Specimen Collection Products

To ensure accurate Nipah virus testing and laboratory safety, MEIDIKE GENE offers a comprehensive range of high-quality specimen collection products suitable for viral diagnostics:

Flocked Oropharyngeal and Nasopharyngeal Swabs

MEIDIKE GENE sterile nylon flocked swabs provide excellent sample absorption and release, making them ideal for RT-PCR testing of Nipah virus and other respiratory pathogens.

病毒转运培养基 (VTM) 套件

MEIDIKE GENE VTM kits are designed to preserve viral RNA integrity during sample transport and storage, supporting reliable molecular testing results.

Complete Specimen Collection Kits

用于实验室, 医院, and public health agencies, MEIDIKE GENE also supplies customized specimen collection kits that include swabs, transport tubes, preservation solutions, and biohazard packaging.

Looking for reliable specimen collection solutions? please contact our sales team at info@medicoswab.com or visit our product page at https://www.medicoswab.com/products/

Prevention and Infection Control Measures

While no specific treatment exists for Nipah virus, prevention focuses on:

• Avoiding contact with infected animals
• 保持良好的手部卫生
• Using PPE in healthcare settings
• Implementing strict laboratory biosafety protocols

Early detection combined with proper specimen collection plays a key role in controlling outbreaks.