Best time to do DNA saliva collection kit test?

Best time to do DNA saliva collection kit test?

ANSWER

30 minutes

It is best to provide a DNA saliva collection kit sample in the morning before eating, drinking, smoking for at least 30 minutes.

This is because food particles and other substances in the mouth can contaminate the sample and reduce the amount of DNA that can be extracted.

Additionally, we recommend rinsing the mouth thoroughly with water and waiting for 10 minutes before collecting the saliva sample.

To avoid reducing the amount of DNA that can extract and affecting hormone/biomarker analyses.

Instruction

It is also important to follow the collection instruction closely, as recommended by www.medicoswab.com.

‘Fill To’ line

Failure to follow the recommendations may result in reduced DNA yield. Some additional tips that can contribute to success include:

Ensuring that subjects deliver the required amount of saliva and that the liquid saliva is at the ‘Fill To’ line; any bubbles present should above the line.

This will ensure that the optimal amount of saliva is enough.
Testing samples from different donors to get an accurate picture of the yields you can expect, as DNA yields will vary between donors.

How long can I keep a saliva collection kit sample before it becomes unusable for DNA testing?

MEIDIKEGENE

According to the research, saliva buffer usually provides stability at room temperature for months or even years.

Genotyping companies like MEIDIKEGENE use saliva collection kits that allow storing the sample up to 2 years.

Additionally, we suggest that specimen should be handed in as soon as possible, and the time consumption should be no more than 7 days.

Therefore, it is best to provide the sample as soon as possible and to send it to the laboratory within a week.

Incubation step

After collection, usually recommends carrying out the 50°C incubation step in the original sample tube before removing an aliquot for DNA extraction.

The incubation only needs to do once; It doesn’t need to rpeate when extracting DNA from subsequent aliquots.