COVID-19 핵산 검사를 위한 올바른 바이러스 보존 솔루션을 선택하는 방법

현재, the form of prevention and control of the new coronavirus is still very severe. As the first step of nucleic acid detection, the importance of sample collection and preservation is undoubted. The inspection industry often says “garbage in, garbage out” is important. The first influencing factor is sample collection. If there is a problem with the sample collection, no matter how well the subsequent work is done, the result will be invalid. Choosing the correct virus preservation solution and collection tube can make the new crown detection more effective

Almost all virus preservation liquids on the market directly preserve live viruses, causing medical staff in sampling, transportation and testing to face a higher risk of infection. In response to this situation, the front line of anti-epidemics is in need of samples that can directly inactivate the virus. 보존 솔루션, but it should be noted that while inactivating the virus, it should also consider whether the preservation solution can stably preserve the integrity of the viral nucleic acid, so as to avoid degradation of the nucleic acid in the sample before the test, resulting in a “거짓 부정” nucleic acid test. Shenzhen Medico does not contain guanidine salt virus preservation solution to minimize the risk of “false negatives”.

We compared the effects of the preservation solution containing guanidine hydrochloride and guanidine isothiocyanate on the market with the Shenzhen Medico preservation solution without guanidine salt in the preservation of poultry coronavirus at 37°C. It turns out that the main ingredient is the preservation solution of guanidine salt, and the preservation effect is very unsatisfactory.

The same conditions are 37°C. After storage for 1-7 날, 바이러스 핵산의 보존 효율은 기본적으로 다음에서 일정합니다. 100% Shenzhen Medico 보존액 사용시; 다른 두 개의 구아니딘 염 성분을 사용하면 바이러스 핵산의 보존 효율이 점차 감소하는 반면. , 7일까지, 그것은 이미 미만이었다 20%, RNA가 시간이 지남에 따라 격렬하게 분해되고 있음을 나타냅니다.

구아니딘염 (구아니딘 이소티오시아네이트 또는 구아니딘 염산염, 등.), 고전적인 단백질 변성제로, 핵산 추출에서 세포 용해에 자주 사용됩니다., and can also achieve the effect of inactivating viruses, but the guanidine salt component does not have the ability to operate under normal temperature conditions. The ability to preserve viral nucleic acid is very easy to cause sample degradation, so it is not suitable for the preservation of new coronavirus samples, so it is strongly recommended that you choose a virus preservation solution that does not contain guanidine salts.

Here, we appeal to the majority of medical workers, 샘플링 목적이 핵산 테스트이고 살아있는 바이러스를 배양할 필요가 없는 경우, 바이러스 보존 솔루션을 선택할 때 다음 측면에주의를 기울여야합니다.:

• 바이러스를 비활성화할 수 있는 보존액 사용

현재 핵산 검출은 샘플에서 바이러스 RNA를 검출하는 것입니다., 바이러스를 용해해야 합니다.. 그러므로, 바이러스 핵산의 무결성과 안정성이 보장되는 한, 살아있는 바이러스를 보존할 필요가 없다. 그러므로, in the current situation of fighting the new coronavirus epidemic, it is more ideal to inactivate the virus while collecting samples. Some manufacturers on the market have also introduced inactivated storage solutions, but the inactivated ingredients used are guanidine salts. The results of comparative experiments found that the guanidine salt component storage solutions do not have the ability to preserve viral nucleic acids under normal temperature conditions, which can easily cause sample degradation. It is not suitable for the preservation of new coronavirus samples. 그러므로, we recommend a virus preservation solution containing non-guanidine salt protein denaturants, which can inactivate the virus and ensure that the viral nucleic acid is not degraded when stored at room temperature.

• The volume of the preservation solution in the sampling tube should be appropriate

It is very important to choose a storage solution with an appropriate volume: (1) If each sample is sampled separately, it is recommended that you choose a virus sampling tube with 1ml storage solution. Not only the sampling volume can meet the needs of downstream nucleic acid extraction and detection, but also compared with 3ml storage solution. The concentration of the virus has increased by 3 타임스! ②If a large-scale initial screening and mixed test is performed, the swab samples of 3-5 people are placed in the same sampling tube. It is recommended that you use a virus sampling tube with 3ml preservation solution to ensure that the sample volume can meet Downstream nucleic acid extraction needs, while improving detection efficiency and reducing detection costs.

• Use a virus preservation solution that can transport and preserve samples at room temperature

Because of the instability of RNA, traditional virus sampling tubes need to be transported to the laboratory within 48 hours at 4°C. If the transport temperature is not up to standard, viral nucleic acid may be degraded, resulting in “거짓 부정” test results! In contrast If Medico uses a virus storage solution that can transport and stably store viral nucleic acids at room temperature, the problem of sample transportation can be solved, thereby improving the accuracy of the test results.

• Use a virus preservation solution that can protect samples from high temperature inactivation

The relevant guidelines of the National Health Commission require high temperature inactivation of the sample before extracting viral nucleic acid (treatment above 56°C for 30 분), but the Beijing Center for Disease Control and Prevention, Beijing Preventive Medicine Research Center and other institutions published research papers in Clinical Chemistry It shows that high temperature inactivation will cause the degradation of viral nucleic acid, thereby reducing the detection rate of viral nucleic acid, which is one of the reasons for the high false negative rate of new coronavirus nucleic acid detection. Experiments have verified that when using Medico virus sample preservation solution to store samples, high temperature inactivation has no significant effect on the integrity of coronavirus nucleic acid.

The virus preservation solutions developed and produced by Shenzhen 의사 are divided into inactivated and non-inactivated types. 다양한 실험 요구 사항 및 테스트 조건에 따라 다양한 보존 솔루션을 선택할 수 있습니다.. 질문이나 필요사항이 있으시면, 문의 전화주세요. Choosing Shenzhen Medico Virus Preservation Solution is your right choice.

그걸 찾아봐: https://www.medicoswab.com/products/transport-medium/virus-transport-medium/