Human Papillomavirus (HPV) testing is crucial for early detection and prevention of cervical cancer. A key factor in accurate HPV testing is the preservation of cellular samples from collection to analysis. This is where the HPV cell preservation solution plays a vital role. In this blog post, we’ll explore the composition, benefits, and practical applications of HPV cell preservation solutions to help you better understand their importance in clinical diagnostics.
What is HPV Cell Preservation Solution?
HPV cell preservation solution is a specially formulated liquid medium used to maintain the integrity of cellular samples collected for HPV testing. After sample collection, the cellular material is suspended in this solution, ensuring the sample remains viable and uncontaminated until laboratory analysis.
Composition of MEIDIKE GENE® HPV Cell Preservation Solution
The effectiveness of MEIDIKE GENE® HPV cell preservation solution lies in its scientifically balanced composition. Its main ingredients include:
- Purified water: Serves as the solvent and diluent, providing a clean, contaminant-free base for the solution.
- Hank’s Balanced Salt Solution (HBSS): Maintains osmotic balance and supplies essential ions to preserve cell structure and function.
- Tween-20: A non-ionic surfactant that helps disperse and suspend cells evenly, preventing clumping and aiding in cell recovery.
- Methanol: Acts as a fixative, preserving cellular morphology and nucleic acids for reliable HPV DNA or RNA analysis.
- Disodium edetate (EDTA): Chelates divalent metal ions, inhibiting nuclease activity and protecting nucleic acids from degradation.
This combination ensures optimal preservation of cellular material and viral DNA for accurate diagnostic testing.
Benefits of Using MEIDIKE GENE®HPV Cell Preservation Solution
Using an effective MEIDIKE GENE® HPV cell preservation solution provides several key advantages:
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DNA Stability: Protects nucleic acids from degradation, ensuring accurate molecular analysis.
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Cell Integrity: Prevents cell clumping and lysing, maintaining sample quality.
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Enhanced Transport Safety: Enables room-temperature sample transportation for short durations.
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Compatible with Diagnostic Platforms: Suitable for PCR-based HPV testing and cytological examinations.
Applications of HPV Preservation Solution
HPV cell preservation solution is widely used in clinical and research settings, particularly for:
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HPV DNA Testing: Preserves the quality of cervical cell samples for HPV screening.
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Cervical Cancer Screening Programs: Used in liquid-based cytology to detect precancerous lesions.
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Molecular Diagnostics: Ensures high-quality nucleic acid samples for downstream testing procedures.
